Differential methylation of the promoter and first exon of the RASSF1A gene in hepatocarcinogenesis.

ABSTRACT:

Aim

Aberrant methylation of the promoter, P2, and the first exon, E1, regions of the tumor suppressor gene RASSF1A, have been associated with hepatocellular carcinoma (HCC), albeit with poor specificity. This study analyzed the methylation profiles of P1, P2 and E1 regions of the gene to identify the region of which methylation most specifically corresponds to HCC and to evaluate the potential of this methylated region as a biomarker in urine for HCC screening.

Methods

Bisulfite DNA sequencing and quantitative methylation-specific polymerase chain reaction assays were performed to compare methylation of the 56 CpG sites in regions P1, P2 and E1 in DNA isolated from normal, hepatitic, cirrhotic, adjacent non-HCC, and HCC liver tissue and urine samples for the characterization

SUBMITTER: Jain S 

PROVIDER: S-EPMC4426255 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature