Validation of housekeeping genes for gene expression analysis in glioblastoma using quantitative real-time polymerase chain reaction.

ABSTRACT:

Background

Quantitative real-time polymerase chain reaction (qPCR) is the most reliable tool for gene expression studies. Selection of housekeeping genes (HKGs) that are having most stable expression is critical to carry out accurate gene expression profiling. There is no 'universal' HKG having stable expression in all kinds of tissues under all experimental conditions.

Methods

The present study aims to identify most appropriate HKGs for gene expression analysis in glioblastoma (GBM) samples. Based on literature survey, six most commonly used HKGs that are invariant in GBM were chosen. We performed qPCR using RNA from formalin fixed paraffin embedded GBM samples and normal brain samples to investigate the expression pattern of HPRT, GAPDH, TBP, B2M, B2M, RPL13A, and RN18S1 w

SUBMITTER: Aithal MG 

PROVIDER: S-EPMC4426273 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature