Genetic Analysis of Substrain Divergence in Non-Obese Diabetic (NOD) Mice.

ABSTRACT: The non-obese diabetic (NOD) mouse is a polygenic model for type 1 diabetes that is characterized by insulitis, a leukocytic infiltration of the pancreatic islets. During ~35 years since the original inbred strain was developed in Japan, NOD substrains have been established at different laboratories around the world. Although environmental differences among NOD colonies capable of impacting diabetes incidence have been recognized, differences arising from genetic divergence have not been analyzed previously. We use both mouse diversity array and whole-exome capture sequencing platforms to identify genetic differences distinguishing five NOD substrains. We describe 64 single-nucleotide polymorphisms, and two short indels that differ in coding regions of the five NOD substrains. A 100-kb del

SUBMITTER: Simecek P 

PROVIDER: S-EPMC4426365 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature