Emergence of homeostatic epithelial packing and stress dissipation through divisions oriented along the long cell axis.

ABSTRACT: Cell division plays an important role in animal tissue morphogenesis, which depends, critically, on the orientation of divisions. In isolated adherent cells, the orientation of mitotic spindles is sensitive to interphase cell shape and the direction of extrinsic mechanical forces. In epithelia, the relative importance of these two factors is challenging to assess. To do this, we used suspended monolayers devoid of ECM, where divisions become oriented following a stretch, allowing the regulation and function of epithelial division orientation in stress relaxation to be characterized. Using this system, we found that divisions align better with the long, interphase cell axis than with the monolayer stress axis. Nevertheless, because the application of stretch induces a global realignment of

SUBMITTER: Wyatt TP 

PROVIDER: S-EPMC4426437 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature