αβ T-cell receptors from multiple sclerosis brain lesions show MAIT cell-related features.

ABSTRACT:

Objectives

To characterize phenotypes of T cells that accumulated in multiple sclerosis (MS) lesions, to compare the lesional T-cell receptor (TCR) repertoire of T-cell subsets to peripheral blood, and to identify paired α and β chains from single CD8(+) T cells from an index patient who we followed for 18 years.

Methods

We combined immunohistochemistry, laser microdissection, and single-cell multiplex PCR to characterize T-cell subtypes and identify paired TCRα and TCRβ chains from individual brain-infiltrating T cells in frozen brain sections. The lesional and peripheral TCR repertoires were analyzed by pyrosequencing.

Results

We found that a TCR Vβ1(+) T-cell population that was strikingly expanded in active brain lesions at clinical onset comprises several subclon

SUBMITTER: Held K 

PROVIDER: S-EPMC4426681 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature