SCL/TAL1-mediated transcriptional network enhances megakaryocytic specification of human embryonic stem cells.

ABSTRACT: Human embryonic stem cells (hESCs) are a unique in vitro model for studying human developmental biology and represent a potential source for cell replacement strategies. Platelets can be generated from cord blood progenitors and hESCs; however, the molecular mechanisms and determinants controlling the in vitro megakaryocytic specification of hESCs remain elusive. We have recently shown that stem cell leukemia (SCL) overexpression accelerates the emergence of hemato-endothelial progenitors from hESCs and promotes their subsequent differentiation into blood cells with higher clonogenic potential. Given that SCL participates in megakaryocytic commitment, we hypothesized that it may potentiate megakaryopoiesis from hESCs. We show that ectopic SCL expression enhances the emergence of megakaryoc

SUBMITTER: Toscano MG 

PROVIDER: S-EPMC4426806 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature