The structure of tubulin-binding cofactor A from Leishmania major infers a mode of association during the early stages of microtubule assembly.

ABSTRACT: Tubulin-binding cofactor A (TBCA) participates in microtubule formation, a key process in eukaryotic biology to create the cytoskeleton. There is little information on how TBCA might interact with β-tubulin en route to microtubule biogenesis. To address this, the protozoan Leishmania major was targeted as a model system. The crystal structure of TBCA and comparisons with three orthologous proteins are presented. The presence of conserved features infers that electrostatic interactions that are likely to involve the C-terminal tail of β-tubulin are key to association. This study provides a reagent and template to support further work in this area.

SUBMITTER: Barrack KL 

PROVIDER: S-EPMC4427162 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature