Sample preparation for phosphoproteomic analysis of circadian time series in Arabidopsis thaliana.

ABSTRACT: Systems biological approaches to study the Arabidopsis thaliana circadian clock have mainly focused on transcriptomics while little is known about the proteome, and even less about posttranslational modifications. Evidence has emerged that posttranslational protein modifications, in particular phosphorylation, play an important role for the clock and its output. Phosphoproteomics is the method of choice for a large-scale approach to gain more knowledge about rhythmic protein phosphorylation. Recent plant phosphoproteomics publications have identified several thousand phosphopeptides. However, the methods used in these studies are very labor-intensive and therefore not suitable to apply to a well-replicated circadian time series. To address this issue, we present and compare different strat

SUBMITTER: Krahmer J 

PROVIDER: S-EPMC4427183 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature