Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams.

ABSTRACT: High-resolution structural information was obtained from lysozyme microcrystals (20 µm in the largest dimension) using raster-scanning serial protein crystallography on micro- and nano-focused beamlines at the ESRF. Data were collected at room temperature (RT) from crystals sandwiched between two silicon nitride wafers, thereby preventing their drying, while limiting background scattering and sample consumption. In order to identify crystal hits, new multi-processing and GUI-driven Python-based pre-analysis software was developed, named NanoPeakCell, that was able to read data from a variety of crystallographic image formats. Further data processing was carried out using CrystFEL, and the resultant structures were refined to 1.7 Å resolution. The data demonstrate the feasibility of RT rast

SUBMITTER: Coquelle N 

PROVIDER: S-EPMC4427202 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature