Identification of two metallothioneins as novel inhalative coffee allergens cof a 2 and cof a 3.

ABSTRACT:

Background

Dust of green coffee beans is known to be a relevant cause for occupational allergic disorders in coffee industry workers. Recently, we described the first coffee allergen (Cof a 1) establishing an allergenic potential of green coffee dust.

Objective

Our aim was to identify allergenic components of green coffee in order to enhance inhalative coffee allergy diagnosis.

Methods

A Coffea arabica pJuFo cDNA phage display library was created and screened for IgE binding with sera from allergic coffee workers. Two further coffee allergens were identified by sequence analysis, expressed in E. coli, and evaluated by Western blots. The prevalence of sensitization to recombinant Cof a 1, Cof a 2, and Cof a 3 and to commercially available extract was investigated by EL

SUBMITTER: Peters U 

PROVIDER: S-EPMC4427360 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature