Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus.

ABSTRACT:

Background

Bacterial pyruvate decarboxylases (PDC) are rare. Their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest. PDCs from Zymomonas mobilis (ZmPDC), Zymobacter palmae (ZpPDC) and Sarcina ventriculi (SvPDC) have been characterized and ZmPDC has been produced successfully in a range of heterologous hosts. PDCs from the Acetobacteraceae and their role in metabolism have not been characterized to the same extent. Examples include Gluconobacter oxydans (GoPDC), G. diazotrophicus (GdPDC) and Acetobacter pasteutrianus (ApPDC). All of these organisms are of commercial importance.

Results

This study reports the kinetic characterization and the crystal structure of a PDC from Gluconacetobacter di

SUBMITTER: van Zyl LJ 

PROVIDER: S-EPMC4428508 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature