Lentiviral vector-based insertional mutagenesis identifies genes involved in the resistance to targeted anticancer therapies.

ABSTRACT: The high transduction efficiency of lentiviral vectors in a wide variety of cells makes them an ideal tool for forward genetics screenings addressing issues of cancer research. Although molecular targeted therapies have provided significant advances in tumor treatment, relapses often occur by the expansion of tumor cell clones carrying mutations that confer resistance. Identification of the culprits of anticancer drug resistance is fundamental for the achievement of long-term response. Here, we developed a new lentiviral vector-based insertional mutagenesis screening to identify genes that confer resistance to clinically relevant targeted anticancer therapies. By applying this genome-wide approach to cell lines representing two subtypes of HER2(+) breast cancer, we identified 62 candidate

SUBMITTER: Ranzani M 

PROVIDER: S-EPMC4429698 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature