Lentiviral arrays for live-cell dynamic monitoring of gene and pathway activity during stem cell differentiation.

ABSTRACT: Uncovering the complexity of mesenchymal stem cell (MSC) differentiation requires novel methods to capture the dynamics of the process in a quantitative and high-throughput manner. To this end, we developed a lentiviral array (LVA) of reporters to capture the dynamics of gene and pathway activity during MSC differentiation into adipogenic, chondrogenic, and osteogenic lineages. Our results identified signature promoters and pathways with unique activation profile for each MSC lineage. In combination with chemical inhibitors, lineage-specific reporters predicted the effects of signaling pathway perturbations on MSC differentiation. Interestingly, some pathways were critical for differentiation into all lineages, while others had differential effects on each lineage. Our study suggests that

SUBMITTER: Padmashali RM 

PROVIDER: S-EPMC4429726 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature