DNase I hypersensitivity analysis of the mouse brain and retina identifies region-specific regulatory elements.

ABSTRACT:

Background

The brain, spinal cord, and neural retina comprise the central nervous system (CNS) of vertebrates. Understanding the regulatory mechanisms that underlie the enormous cell-type diversity of the CNS is a significant challenge. Whole-genome mapping of DNase I-hypersensitive sites (DHSs) has been used to identify cis-regulatory elements in many tissues. We have applied this approach to the mouse CNS, including developing and mature neural retina, whole brain, and two well-characterized brain regions, the cerebellum and the cerebral cortex.

Results

For the various regions and developmental stages of the CNS that we analyzed, there were approximately the same number of DHSs; however, there were many DHSs unique to each CNS region and developmental stage. Many of the DH

SUBMITTER: Wilken MS 

PROVIDER: S-EPMC4429822 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature