Deep Sequencing Analysis of the Ixodes ricinus Haemocytome.

ABSTRACT:

Background

Ixodes ricinus is the main tick vector of the microbes that cause Lyme disease and tick-borne encephalitis in Europe. Pathogens transmitted by ticks have to overcome innate immunity barriers present in tick tissues, including midgut, salivary glands epithelia and the hemocoel. Molecularly, invertebrate immunity is initiated when pathogen recognition molecules trigger serum or cellular signalling cascades leading to the production of antimicrobials, pathogen opsonization and phagocytosis. We presently aimed at identifying hemocyte transcripts from semi-engorged female I. ricinus ticks by mass sequencing a hemocyte cDNA library and annotating immune-related transcripts based on their hemocyte abundance as well as their ubiquitous distribution.

Methodology/principal find

SUBMITTER: Kotsyfakis M 

PROVIDER: S-EPMC4430169 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature