Granulosa cell-derived induced pluripotent stem cells exhibit pro-trophoblastic differentiation potential.

ABSTRACT:

Introduction

Human induced pluripotent stem cells (hiPSCs) have been derived from various somatic cell types. Granulosa cells, a group of cells which surround oocytes and are obtained from the (normally discarded) retrieved egg follicles of women undergoing infertility treatment, are a possible cell source for induced pluripotent stem cell (iPSC) generation. Here, we explored the possibility of using human granulosa cells as a donor cell type for iPSC reprogramming, and compared granulosa cell-derived iPSCs (iGRAs) with those derived from other cell sources, to determine the potential ability of iGRA differentiation.

Methods

Granulosa cells were collected from egg follicles retrieved from women undergoing infertility treatment. After short-term culture, the granulosa cells d

SUBMITTER: Chuang CY 

PROVIDER: S-EPMC4430911 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature