Differential Genes Expression between Fertile and Infertile Spermatozoa Revealed by Transcriptome Analysis.

ABSTRACT:

Background

It was believed earlier that spermatozoa have no traces of RNA because of loss of most of the cytoplasm. Recent studies have revealed the presence of about 3000 different kinds of mRNAs in ejaculated spermatozoa. However, the correlation of transcriptome profile with infertility remains obscure.

Methods

Total RNA from sperm (after exclusion of somatic cells) of 60 men consisting of individuals with known fertility (n=20), idiopathic infertility (normozoospermic patients, n=20), and asthenozoospermia (n=20) was isolated. After RNA quality check on Bioanalyzer, AffymetrixGeneChip Human Gene 1.0 ST Array was used for expression profiling, which consisted of >30,000 coding transcripts and >11,000 long intergenic non-coding transcripts.

Results

Comparison betwee

SUBMITTER: Bansal SK 

PROVIDER: S-EPMC4431685 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature