Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems.

ABSTRACT:

Background

Rapid, accurate and high-throughput identification of vector arthropods is of paramount importance in surveillance programmes that are becoming more common due to the changing geographic occurrence and extent of many arthropod-borne diseases. Protein profiling by MALDI-TOF mass spectrometry fulfils these requirements for identification, and reference databases have recently been established for several vector taxa, mostly with specimens from laboratory colonies.

Methods

We established and validated a reference database containing 20 phlebotomine sand fly (Diptera: Psychodidae, Phlebotominae) species by using specimens from colonies or field-collections that had been stored for various periods of time.

Results

Identical biomarker mass patterns ('superspectra

SUBMITTER: Mathis A 

PROVIDER: S-EPMC4432514 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature