Liver injury and fibrosis induced by dietary challenge in the Ossabaw miniature Swine.

ABSTRACT:

Background

Ossabaw miniature swine when fed a diet high in fructose, saturated fat and cholesterol (NASH diet) develop metabolic syndrome and nonalcoholic steatohepatitis (NASH) characterized by liver injury and fibrosis. This study was conducted to further characterize the development of NASH in this large animal model.

Methods

Ossabaw swine were fed standard chow (control group; n = 6) or NASH diet (n = 6) for 24 weeks. Blood and liver tissue were collected and liver histology were characterized at 0, 8, 16 and 24 weeks of dietary intervention. Hepatic apoptosis and lipid levels were assessed at week 24.

Results

The NASH diet group developed metabolic syndrome and progressive histologic features of NASH including: (a) hepatocyte ballooning at 8 weeks which progresse

SUBMITTER: Liang T 

PROVIDER: S-EPMC4433111 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature