The Intraperitoneal Transcriptome of the Opportunistic Pathogen Enterococcus faecalis in Mice.

ABSTRACT: Enterococcus faecalis is a Gram-positive lactic acid intestinal opportunistic bacterium with virulence potential. For a better understanding of the adapation of this bacterium to the host conditions, we performed a transcriptome analysis of bacteria isolated from an infection site (mouse peritonitis) by RNA-sequencing. We identified a total of 211 genes with significantly higher transcript levels and 157 repressed genes. Our in vivo gene expression database reflects well the infection process since genes encoding important virulence factors like cytolysin, gelatinase or aggregation substance as well as stress response proteins, are significantly induced. Genes encoding metabolic activities are the second most abundant in vivo induced genes demonstrating that the bacteria are metabolically

SUBMITTER: Muller C 

PROVIDER: S-EPMC4433114 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature