Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation.

ABSTRACT: Comprehensive analysis of alterations in gene expression along with neoplastic transformation in human cells provides valuable information about the molecular mechanisms underlying transformation. To further address these questions, we performed whole transcriptome analysis to the human mesenchymal stem cell line, UE6E7T-3, which was immortalized with hTERT and human papillomavirus type 16 E6/E7 genes, in association with progress of transformation in these cells. At early stages of culture, UE6E7T-3 cells preferentially lost one copy of chromosome 13, as previously described; in addition, tumor suppressor genes, DNA repair genes, and apoptosis-activating genes were overexpressed. After the loss of chromosome 13, additional aneuploidy and genetic alterations that drove progressive transfor

SUBMITTER: Takeuchi M 

PROVIDER: S-EPMC4433180 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature