Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay.

ABSTRACT: Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical noro

SUBMITTER: Hagstrom AE 

PROVIDER: S-EPMC4433186 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature