Differential levels of Neurod establish zebrafish endocrine pancreas cell fates.

ABSTRACT: During development a network of transcription factors functions to differentiate foregut cells into pancreatic endocrine cells. Differentiation of appropriate numbers of each hormone-expressing endocrine cell type is essential for the normal development of the pancreas and ultimately for effective maintenance of blood glucose levels. A fuller understanding of the details of endocrine cell differentiation may contribute to development of cell replacement therapies to treat diabetes. In this study, by using morpholino and gRNA/Cas9 mediated knockdown we establish that differential levels of the basic-helix loop helix (bHLH) transcription factor Neurod are required for the differentiation of distinct endocrine cell types in developing zebrafish. While Neurod plays a role in the differentiatio

SUBMITTER: Dalgin G 

PROVIDER: S-EPMC4433608 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature