Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis.

ABSTRACT:

Background

Sialic acid-binding, immunoglobulin-like lectin (Siglec) F is a glycan-binding protein selectively expressed on mouse eosinophils. Its engagement induces apoptosis, suggesting a pathway for ameliorating eosinophilia in the setting of asthma and other eosinophil-associated diseases. Siglec-F recognizes sialylated sulfated glycans in glycan-binding assays, but the identities of endogenous sialoside ligands and their glycoprotein carriers in vivo are unknown.

Objectives

To use mouse lung-derived materials to isolate, biochemically identify, and biologically characterize naturally occurring endogenous glycan ligands for Siglec-F.

Methods

Lungs from normal and mucin-deficient mice, as well as mouse tracheal epithelial cells, were investigated in vitro and in viv

SUBMITTER: Kiwamoto T 

PROVIDER: S-EPMC4433759 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature