Expression, Clinical Significance, and Receptor Identification of the Newest B7 Family Member HHLA2 Protein.

ABSTRACT:

Purpose

HHLA2 (B7H7/B7-H5/B7y) is a newly identified B7 family member that regulates human T-cell functions. However, its protein expression in human organs and significance in human diseases are unknown. The objective of this study was to analyze HHLA2 protein expression in normal human tissues and cancers, as well as its prognostic significance, to explore mechanisms regulating HHLA2 expression, and to identify candidate HHLA2 receptors.

Experimental design

An immunohistochemistry protocol and a flow cytometry assay with newly generated monoclonal antibodies were developed to examine HHLA2 protein. HHLA2 gene copy-number variation was analyzed from cancer genomic data. The combination of bioinformatics analysis and immunologic approaches was established to explore HHLA2 re

SUBMITTER: Janakiram M 

PROVIDER: S-EPMC4433806 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature