Colorectal cancer severity and survival in correlation with tumour necrosis factor-alpha.

ABSTRACT: Colorectal cancer (CRC) development is strongly associated with innate immune mechanisms and intestinal inflammation. The aim of the study was to investigate the pre-operative serum levels of TNF-α and its correlation with cancer progression and survival in CRC patients taking into account the genotype of -308G/A promoter polymorphism in TNF-α gene (rs1800629). TNF-α -308G/A genotypes of 119 CRC cases and 177 no CRC controls were determined by restriction fragment length polymorphism assay (RFLP-PCR). TNF-α serum levels were measured by enzyme-linked immunosorbent assay (ELISA). Although no significant differences in allele and genotype frequencies between CRC and controls were observed, it should be noted that the minor allele-A and its homozygous genotype were overrepresented among CRC.

SUBMITTER: Stanilov N 

PROVIDER: S-EPMC4433886 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature