Molecular detection of bacteria in plant tissues, using universal 16S ribosomal DNA degenerated primers.

ABSTRACT: Highly specific, sensitive and rapid tests are required for the detection and identification of covert bacterial contaminations in plant tissue cultures. Current methods available for this purpose are tedious, time consuming, highly error prone, expensive, require advanced technical expertise and are sometimes ineffective. We report here the development of a sensitive polymerase chain reaction (PCR) based method for the rapid detection and identification of bacteria occurring in plant tissue cultures. A total of 121 16S ribosomal DNA (rDNA) coding regions from 14 different groups of bacteria, algae and plants, available in the Gene Bank/European Molecular Biology Laboratory databases, were aligned and several conserved DNA sequences of bacterial origin were identified. From those, five deg

SUBMITTER: Tsoktouridis G 

PROVIDER: S-EPMC4434121 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature