Structures to complement the archaeo-eukaryotic primases catalytic cycle description: What's next?

ABSTRACT: DNA replication is a crucial stage in the transfer of genetic information from parent to daughter cells. This mechanism involves multiple proteins with one key player being the primase. Primases are single-stranded DNA dependent RNA polymerases. On the leading strand, they synthesize the primer once allowing DNA elongation while on the lagging strand primers are generated repeatedly (Okazaki fragments). Primases have the unique ability to create the first phosphodiester bond yielding a dinucleotide which is initially elongated by primases and then by DNA polymerases. Primase activity has been studied in the last decades but the detailed molecular steps explaining some unique features remain unclear. High-resolution structures of free and bound primases domains have brought significant insi

SUBMITTER: Boudet J 

PROVIDER: S-EPMC4434180 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature