CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture.

ABSTRACT: Compensatory growth is mediated by multiple cell types that interact during organ repair. To elucidate the relationship between stem/progenitor cells that proliferate or differentiate and somatic cells of the lung, we used a novel organotypic ex vivo pneumoexplant system. Applying this technique, we identified a sustained culture of repopulating adult progenitors in the form of free-floating anchorage-independent cells (AICs). AICs did not express integrin proteins α5, β3 and β7, and constituted 37% of the total culture at day 14, yielding a mixed yet conservative population that recapitulated RNA expression patterns of the healthy lung. AICs exhibited rapid proliferation manifested by a marked 60-fold increase in cell numbers by day 21. More than 50% of the AIC population was c-KIT(+) or

SUBMITTER: Peter Y 

PROVIDER: S-EPMC4434406 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature