Identification and characterisation of putative seminal fluid proteins from male reproductive tissue EST libraries in tiger beetles.

ABSTRACT:

Background

The study of proteins transferred through semen can provide important information for biological questions such as adaptive evolution, the origin of new species and species richness. The objective of this study was to identify seminal fluid proteins (SFPs) that may contribute to the study of the reproductive system of tiger beetles (cicindelids), a group of more than 2,500 species distributed worldwide that occupy a great diversity of habitats.

Results

Two cDNA libraries were constructed from the male gonads of Calomera littoralis and Cephalota litorea. Expressed sequence tags (ESTs) were analysed by bioinformatics approaches and 14 unigenes were selected as candidate SFPs, which were submitted to Reverse Transcription Polymerase Chain Reaction (RT-PCR) to identif

SUBMITTER: Rodriguez-Garcia MJ 

PROVIDER: S-EPMC4434525 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature