Comparison of the three-dimensional organization of sperm and fibroblast genomes using the Hi-C approach.

ABSTRACT:

Background

The three-dimensional organization of the genome is tightly connected to its biological function. The Hi-C approach was recently introduced as a method that can be used to identify higher-order chromatin interactions genome-wide. The aim of this study was to determine genome-wide chromatin interaction frequencies using the Hi-C approach in mouse sperm cells and embryonic fibroblasts.

Results

The obtained data demonstrate that the three-dimensional genome organizations of sperm and fibroblast cells show a high degree of similarity both with each other and with the previously described mouse embryonic stem cells. Both A- and B-compartments and topologically associated domains are present in spermatozoa and fibroblasts. Nevertheless, sperm cells and fibroblasts exhib

SUBMITTER: Battulin N 

PROVIDER: S-EPMC4434584 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature