Characterizing the in vivo role of trehalose in Saccharomyces cerevisiae using the AGT1 transporter.

ABSTRACT: Trehalose is a highly stable, nonreducing disaccharide of glucose. A large body of research exists implicating trehalose in a variety of cellular phenomena, notably response to stresses of various kinds. However, in very few cases has the role of trehalose been examined directly in vivo. Here, we describe the development and characterization of a system in Saccharomyces cerevisiae that allows us to manipulate intracellular trehalose concentrations independently of the biosynthetic enzymes and independently of any applied stress. We found that many physiological roles heretofore ascribed to intracellular trehalose, including heat resistance, are not due to the presence of trehalose per se. We also found that many of the metabolic and growth defects associated with mutations in the trehalose

SUBMITTER: Gibney PA 

PROVIDER: S-EPMC4434743 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature