Transcriptomic diversification of developing cumulus and mural granulosa cells in mouse ovarian follicles.

ABSTRACT: Cumulus cells and mural granulosa cells (MGCs) have functionally distinct roles in antral follicles, and comparison of their transcriptomes at a global and systems level can propel future studies on mechanisms underlying their functional diversity. These cells were isolated from small and large antral follicles before and after stimulation of immature mice with gonadotropins, respectively. Both cell types underwent dramatic transcriptomic changes, and differences between them increased with follicular growth. Although cumulus cells of both stages of follicular development are competent to undergo expansion in vitro, they were otherwise remarkably dissimilar with transcriptomic changes quantitatively equivalent to those of MGCs. Gene ontology analysis revealed that cumulus cells of small fo

SUBMITTER: Wigglesworth K 

PROVIDER: S-EPMC4434932 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature