Single-cell Migration Chip for Chemotaxis-based Microfluidic Selection of Heterogeneous Cell Populations.

ABSTRACT: Tumor cell migration toward and intravasation into capillaries is an early and key event in cancer metastasis, yet not all cancer cells are imbued with the same capability to do so. This heterogeneity within a tumor is a fundamental property of cancer. Tools to help us understand what molecular characteristics allow a certain subpopulation of cells to spread from the primary tumor are thus critical for overcoming metastasis. Conventional in vitro migration platforms treat populations in aggregate, which leads to a masking of intrinsic differences among cells. Some migration assays reported recently have single-cell resolution, but these platforms do not provide for selective retrieval of the distinct migrating and non-migrating cell populations for further analysis. Thus, to study the intr

SUBMITTER: Chen YC 

PROVIDER: S-EPMC4435023 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature