Post-ovulatory ageing and egg quality: a proteomic analysis of rainbow trout coelomic fluid.

ABSTRACT:

Background

In fish, oocyte post-ovulatory ageing is associated with egg quality decrease. During this period, eggs are held in the body cavity where they bath in a semi-viscous liquid known as coelomic fluid (CF). CF components are suspected to play a role in maintaining oocyte fertility and developmental competence (egg quality). However, CF proteic composition remains poorly studied. Thus rainbow trout CF proteome was studied during the egg quality decrease associated with oocyte post-ovulatory ageing.

Methods

High resolution two-dimensional gel electrophoresis was used to analyze the proteome of rainbow trout (Oncorhynchus mykiss) CF in relationship with the egg quality decrease associated with oocyte post-ovulatory ageing. A first experiment was performed using CF pools

SUBMITTER: Rime H 

PROVIDER: S-EPMC443514 | biostudies-literature | 2004 Jun

REPOSITORIES: biostudies-literature