Development of microsatellite primers in the protected species Viola elatior (Violaceae) using next-generation sequencing.

ABSTRACT: Viola elatior (Violaceae) is a Eurasian perennial plant species in which French populations are threatened by anthropogenic pressures. Microsatellite primers were developed to investigate its genetic structure and diversity. Eight microsatellite markers were isolated using next-generation sequencing. Loci were amplified and screened for 138 individuals in 17 populations from France. Two of the eight polymorphic loci presented no variability across populations. The total number of alleles per locus varied from two to four. Observed heterozygosity ranged from 0.051 to 1.000. All primers amplified successfully in the closely related species V. pumila. This set of microsatellites offers a valuable tool for assessing population genetic diversity of the species to improve its conservation and ba

SUBMITTER: Celik M 

PROVIDER: S-EPMC4435467 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature