Development of 23 polymorphic microsatellite loci in invasive silver wattle, Acacia dealbata (Fabaceae).

ABSTRACT: Microsatellite markers were developed for silver wattle, Acacia dealbata (Fabaceae), which is both an ornamental and an invasive weed species. It is native to southeastern Australia and invasive in Europe, Africa, Asia, and the Americas. The pyrosequencing of a microsatellite-enriched genomic DNA library of A. dealbata produced 33,290 sequences and allowed the isolation of 201 loci with a minimum of seven repeats of microsatellite motifs. Amplification tests led to the setup of two multiplex PCR mixes allowing the amplification of 21 loci. The polymorphism of these markers was evaluated on a sample of 32 individuals collected in southeastern Australia. The number of alleles and the expected heterozygosity varied between two and 11, and between 0.11 and 0.88, respectively. The level of poly

SUBMITTER: Guillemaud T 

PROVIDER: S-EPMC4435469 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature