Gene and MicroRNA Expression Responses to Exercise; Relationship with Insulin Sensitivity.

ABSTRACT:

Background

Healthy individuals on the lower end of the insulin sensitivity spectrum also have a reduced gene expression response to exercise for specific genes. The goal of this study was to determine the relationship between insulin sensitivity and exercise-induced gene expression in an unbiased, global manner.

Methods and findings

Euglycemic clamps were used to measure insulin sensitivity and muscle biopsies were done at rest and 30 minutes after a single acute exercise bout in 14 healthy participants. Changes in mRNA expression were assessed using microarrays, and miRNA analysis was performed in a subset of 6 of the participants using sequencing techniques. Following exercise, 215 mRNAs were changed at the probe level (Bonferroni-corrected P<0.00000115). Pathway and Gene

SUBMITTER: McLean CS 

PROVIDER: S-EPMC4436215 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature