Characterization of the proteome of cytoplasmic lipid droplets in mouse enterocytes after a dietary fat challenge.

ABSTRACT: Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around t

SUBMITTER: D'Aquila T 

PROVIDER: S-EPMC4436333 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature