QuantiFly: Robust Trainable Software for Automated Drosophila Egg Counting.

ABSTRACT: We report the development and testing of software called QuantiFly: an automated tool to quantify Drosophila egg laying. Many laboratories count Drosophila eggs as a marker of fitness. The existing method requires laboratory researchers to count eggs manually while looking down a microscope. This technique is both time-consuming and tedious, especially when experiments require daily counts of hundreds of vials. The basis of the QuantiFly software is an algorithm which applies and improves upon an existing advanced pattern recognition and machine-learning routine. The accuracy of the baseline algorithm is additionally increased in this study through correction of bias observed in the algorithm output. The QuantiFly software, which includes the refined algorithm, has been designed to be imme

SUBMITTER: Waithe D 

PROVIDER: S-EPMC4436334 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature