Isolation and characterisation of mesenchymal stem/stromal cells in the ovine endometrium.

ABSTRACT:

Objective

Mesenchymal stem/stromal cells (MSC) were recently discovered in the human endometrium. These cells possess key stem cell properties and show promising results in small animal models when used for preclinical tissue engineering studies. A small number of surface markers have been identified that enrich for MSC from bone marrow and human endometrium, including the Sushi Domain-containing 2 (SUSD2; W5C5) and CD271 markers. In preparation for developing a large animal preclinical model for urological and gynecological tissue engineering applications we aimed to identify and characterise MSC in ovine endometrium and determine surface markers to enable their prospective isolation.

Materials and methods

Ovine endometrium was obtained from hysterectomised ewes following p

SUBMITTER: Letouzey V 

PROVIDER: S-EPMC4436363 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature