Microtubule-dependent transport and dynamics of vimentin intermediate filaments.

ABSTRACT: We studied two aspects of vimentin intermediate filament dynamics-transport of filaments and subunit exchange. We observed transport of long filaments in the periphery of cells using live-cell structured illumination microscopy. We studied filament transport elsewhere in cells using a photoconvertible-vimentin probe and total internal reflection microscopy. We found that filaments were rapidly transported along linear tracks in both anterograde and retrograde directions. Filament transport was microtubule dependent but independent of microtubule polymerization and/or an interaction with the plus end-binding protein APC. We also studied subunit exchange in filaments by long-term imaging after photoconversion. We found that converted vimentin remained in small clusters along the length of fi

SUBMITTER: Hookway C 

PROVIDER: S-EPMC4436779 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature