The newt (Cynops pyrrhogaster) RPE65 promoter: molecular cloning, characterization and functional analysis.

ABSTRACT: The adult newt has the ability to regenerate the neural retina following injury, a process achieved primarily by the retinal pigment epithelium (RPE). To deliver exogenous genes to the RPE for genetic manipulation of regenerative events, we isolated the newt RPE65 promoter region by genome walking. First, we cloned the 2.8 kb RPE65 promoter from the newt, Cynops pyrrhogaster. Sequence analysis revealed several conserved regulatory elements described previously in mouse and human RPE65 promoters. Second, having previously established an I-SceI-mediated transgenic protocol for the newt, we used it here to examine the -657 bp proximal promoter of RPE65. The promoter assay used with F0 transgenic newts confirmed transgene expression of mCherry fluorescent protein in the RPE. Using bioinformati

SUBMITTER: Casco-Robles MM 

PROVIDER: S-EPMC4436847 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature