Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra.

ABSTRACT:

Background

The hallmark of tuberculosis is the granuloma, an organized cellular accumulation playing a key role in host defense against Mycobacterium tuberculosis. These structures sequester and contain mycobacterial cells preventing active disease, while long term maintenance of granulomas leads to latent disease. Clear understanding on mechanisms involved in granuloma formation and maintenance is lacking.

Objective

To monitor granuloma formation and to determine gene expression profiles induced during the granulomatous response to M. tuberculosis (H37Ra).

Methods

We used a previously characterized in vitro human model. Cellular aggregation was followed daily with microscopy and Wright staining for 5 days. Granulomas were collected at 24 h, RNA extracted and hybridiz

SUBMITTER: Reyes N 

PROVIDER: S-EPMC4437284 | biostudies-literature | 2015 Jan-Mar

REPOSITORIES: biostudies-literature