Classifying patients for breast cancer by detection of autoantibodies against a panel of conformation-carrying antigens.

ABSTRACT: Patients with breast cancer elicit an autoantibody response against cancer proteins, which reflects and amplifies the cellular changes associated with tumorigenesis. Detection of autoantibodies in plasma may provide a minimally invasive mechanism for early detection of breast cancer. To identify cancer proteins that elicit a humoral response, we generated a cDNA library enriched for breast cancer genes that encode membrane and secreted proteins, which are more likely to induce an antibody response compared with intracellular proteins. To generate conformation-carrying antigens that are efficiently recognized by patients' antibodies, a eukaryotic expression strategy was established. Plasma from 200 patients with breast cancer and 200 age-matched healthy controls were measured for autoantibo

SUBMITTER: Evans RL 

PROVIDER: S-EPMC4437507 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature