Serine effects on collision-induced dissociation and photodissociation of peptide cation radicals of the z^+• -type.

ABSTRACT: The serine residue displays specific effects on the dissociations of peptide fragment cation-radicals of the z^+• type which are produced by electron transfer dissociation. Energy-resolved collision-induced dissociation (ER-CID), time-resolved infrared multiphoton dissociation (TR-IRMPD), and single-photon UV photodissociation at 355 nm revealed several competitive dissociation pathways consisting of loss of OH radical, water, and backbone cleavages occurring at N-terminal and C-terminal positions relative to the serine residue. The activation modes using slow-heating and UV photon absorption resulted in different relative intensities of fragment ions. This indicated that the dissociations proceeded through several channels with different energy-depend

SUBMITTER: Nguyen HT 

PROVIDER: S-EPMC4437545 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature