Electrophoretic Techniques for the Detection of Human Microsatellite D19S884.

ABSTRACT: BACKGROUND:The detection and analysis of microsatellites is very important for the mapping of genetic diseases because they are commonly used as genetic markers. Microsatellite marker D19S884 has been associated with polycystic ovary syndrome (PCOS), the most common reproductive endocrine disease of women in their childbearing years. It is responsible for an estimated 70% of cases of anovulatory infertility. In this work, we detected microsatellites in DNA extracted from the blood of PCOS patients. METHODS:DNA microsatellites were amplified by polymerase chain reaction (PCR) using a pair of specific primers tagged with fluorescence to yield products of 160-200 base pairs in length. Alleles were separated on 4% low-melting agarose gels; stained with a safe gel staining, GelRed™, which is an

SUBMITTER: Mansor F 

PROVIDER: S-EPMC4438088 | biostudies-literature | 2015 Mar-Apr

REPOSITORIES: biostudies-literature