Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype.

ABSTRACT:

Background

Functional brown adipose tissue (BAT), involved in energy expenditure, has recently been detected in substantial amounts in adults. Formerly overlooked BAT has now become an attractive anti-obesity target.

Methods and results

Molecular characterization of human brown and white adipocytes, using a myriad of techniques including high-throughput RNA sequencing and functional assays, showed that PAZ6 and SW872 cells exhibit classical molecular and phenotypic markers of brown and white adipocytes, respectively. However, the pre-adipocyte cell line SGBS presents a versatile phenotype. A transit expression of classical brown markers such as UCP1 and PPARγ peaked and declined at day 28 post-differentiation initiation. Conversely, white adipocyte markers, including Tcf21,

SUBMITTER: Guennoun A 

PROVIDER: S-EPMC4438513 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature