The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis.

ABSTRACT: Dynamic remodeling of the actin cytoskeleton is required for both development and tissue homeostasis. While fixed image analysis has provided significant insight into such events, a complete understanding of cytoskeletal dynamics requires live imaging. Numerous tools for the live imaging of actin have been generated by fusing the actin-binding domain from an actin-interacting protein to a fluorescent protein. Here we comparatively assess the utility of three such tools--Utrophin, Lifeact, and F-tractin--for characterizing the actin remodeling events occurring within the germline-derived nurse cells during Drosophila mid-oogenesis or follicle development. Specifically, we used the UAS/GAL4 system to express these tools at different levels and in different cells, and analyzed these tools for

SUBMITTER: Spracklen AJ 

PROVIDER: S-EPMC4438707 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature