Improved recellularization of ex vivo vascular scaffolds using directed transport gradients to modulate ECM remodeling.

ABSTRACT: The regeneration of functional, clinically viable, tissues from acellular ex vivo tissues has been problematic largely due to poor nutrient transport conditions that limit cell migration and integration. Compounding these issues are subcellular pore sizes that necessarily requires extracellular matrix (ECM) remodeling in order for cells to migrate and regenerate the tissue. The aim of the present work was to create a directed growth environment that allows cells to fully populate an ex vivo-derived vascular scaffold and maintain viability over extended periods. Three different culture conditions using single (one nutrient source) or dual perfusion bioreactor systems (two nutrients sources) were designed to assess the effect of pressure and nutrient gradients under either low (50/30 mmHg) o

SUBMITTER: Tosun Z 

PROVIDER: S-EPMC4438987 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature